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. 2017 Nov 24;8:1618. doi: 10.3389/fimmu.2017.01618

Figure 5.

Figure 5

Expression of rtTA and administration of doxycycline fails to activate tet-On genes in resident alveolar macrophages (ResAM). CD68-rtTA and R26-rtTA reporter mice were administered doxycycline for 10 months, then reporter expression was examined in the lung. (A) Reporter expression in ResAM and interstitial macrophages (IM) after 10 months of doxycycline administration. (B) Representative histograms of ResAM (red) and IM (blue) from CD68-rtTA reporter mice overlaid on ResAM from non-reporter control animals (gray); dot plot of ResAM (red) and IM (blue) from CD68-rtTA reporter mice overlaid on all other CD45+ cells (gray) from R26-rtTA reporter mice. (C) Representative histograms of ResAM (red) and IM (blue) from R26-rtTA reporter mice overlaid on ResAM from non-reporter control animals (gray); dot plot of ResAM (red) and IM (blue) from R26-rtTA reporter mice overlaid on all other CD45+ cells (gray) from R26-rtTA reporter mice. Data are presented as mean ± SD, n = 4–5 mice per group.