GSK-3β inhibition increases mRNA expression of β-catenin and osteogenic markers in both the tibial bone underneath the implant and intact femur. Relative mRNA expression of β-catenin (A, F), Runx2 (B, G), Osterix (C, H), ALP (D, I) and Col1α1 (E, J) in tibial bone subjected to instability (upper panels) and intact femoral shaft (lower panels). Results are presented as the fold-change in tibial mRNA values compared to non-loaded controls (0 days) in bone harvested from animals subjected to instability or femoral mRNA in vehicle-treated animals. Graphs indicate mean±SD in each group. a: significant compared to vehicle-treated animals, b: significant changes compared to controls. A p-value of <0.05 was considered significant.