Table 1.
No. of animals | No. of samples for each analysis | |||||
---|---|---|---|---|---|---|
| ||||||
Group | Total | qPCR | μCT | IHC* | ELISA** | |
Implant instability (Tibia) | Control | 15 | 7 | 8 | 6† | 11 |
Vehicle (d3) | 15 | 7 | – | 8 | 11 | |
AR28 (d3) | 15 | 6† | – | 8 | 12 | |
Vehicle (d5) | 15 | 7 | 8 | 8 | 11 | |
AR28 (d5) | 15 | 4† | 8 | 7† | 12 | |
| ||||||
Intact animals (Femur) | Vehicle (d5) | 16 | 8 | 8 | 7† | 11 |
AR28 (d5) | 16 | 8 | 8 | 8 | 12 |
qPCR: quantitative polymerase chain reaction; μCT: micro-computed tomography; IHC: Immunohistochemistry; ELISA: Enzyme-linked immunosorbent assay.
: Samples used for μCT and IHC analyses were collected from the same animals.
: Blood samples used for ELISA were collected randomly from animals in IHC and qPCR groups.
: Samples excluded due to technical problems during sample processing.