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. 2017 Nov 28;7:16530. doi: 10.1038/s41598-017-16561-5

Figure 6.

Figure 6

Effects of capric acid treatment on intestinal permeability after cyclophosphamide treatment in IPEC-J2 cells. (a) Membrane FD-4 permeability of cyclophosphamide-induced IPEC-J2 cells with or without capric acid pre-treatment. Membrane permeability was quantitated by measuring the amount of FD-4 that permeated the IPEC-J2 monolayer. (b) Relative quantitative expression of the genes encoding tight junctions (ZO-1 and OCLN) for capric acid treatment after cyclophosphamide treatment in IPEC-J2 cells. The qRT-PCR data were normalised to the expression of GAPDH as an endogenous control gene and calculated using the 2−ΔΔCt method. Error bars indicate the standard error of the mean (n = 3). A p-value of <0.05 indicated statistical significance. Lowercase letters (a, b, c) indicate significant differences between treatments based on Duncan multiple range tests.