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. 2017 Nov 28;7:16530. doi: 10.1038/s41598-017-16561-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative quantitative expression of genes encoding (a) pro-inflammatory cytokines (TNF-α, IFN-γ, and IL-6) and (b) anti-inflammatory cytokines (IL-4 and IL-10) for capric acid treatment after cyclophosphamide challenge in peripheral blood mononuclear cells of miniature pigs. Miniature pigs were randomly allocated into three groups: (T1) control diet + saline challenge; (T2) control diet + CTX challenge; and (T3) control diet with 0.5% capric acid + CTX challenge. The qRT-PCR data were normalised relative to the expression of GAPDH as an endogenous control gene and calculated using the 2^−ΔΔCt method. Error bars indicate the standard error of the mean. A p-value of < 0.05 was considered to indicate statistical significance. Lowercase letters (a, b, c) indicate significant differences between treatments based on Duncan multiple range tests.