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. 2017 Nov 28;7:16475. doi: 10.1038/s41598-017-16593-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tetrac, triac and T1AM affect apoptotic gene expression. Cells were treated for 1–4 h with 10 or 25 μM of (A) tetrac, (B) triac (C) T1AM and mRNA levels were assessed by real-time PCR. Results were calculated as fold change using the comparative threshold cycle method (2-ΔΔCT) relative to control cells (that is, controls are arbitrarily assigned a value of 1). Experiments were repeated three times and are presented (average ± STDEV) as fold of control (1 ± STDEV, dashed line). *p < 0.05, **<0.005.