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. 2017 Nov 28;7:16462. doi: 10.1038/s41598-017-16755-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of temperature on the interaction mode of CheA and CheY. (a) Overlaid ¹H-¹³C HMQC spectra of the [u-²H, Ileδ1-¹³CH₃, Metε-¹³CH₃, Alaβ-¹³CH₃]-P1/[²H]-P2–5 CheA in the presence of various amounts of non-labeled CheY, observed at 283 K. Only the regions with the I5, I50, I87, and I119 resonances are shown. The centers of the I5 resonances are indicated with dots, and the amounts of CheY relative to CheA are indicated. (b,c) Plots of the ¹H (b) and ¹³C (c) chemical shifts of the resonances from I5, observed at 303 K (black circles, shown in Fig. 1e,f) or 283 K (white circles), against the relative amounts of CheY. Error bars represent standard deviations of the chemical shifts of 100 synthetic time domain data from the in situ error analysis⁸⁴.