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. 2017 Nov 28;7:16462. doi: 10.1038/s41598-017-16755-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature-dependent shift of the equilibrium of the CheA-CheY complex. (a,b) Estimated equilibrium constants and their standard deviations at 303 K (a) and 283 K (b), determined by the Markov chain Monte Carlo algorithm. (c,d) are overlaid resonances from the methyl group of I5 of CheA, in the presence of various concentrations of CheY, simulated using the equilibrium constants in (a,b), respectively, with the same colors as in Figs 1d and 2a.