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. 2017 Nov 28;7:16462. doi: 10.1038/s41598-017-16755-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-linear shift of the resonances from M3. (a) Overlaid ¹H-¹³C HMQC spectra of [u-²H, Ileδ₁-¹³CH₃, Metε-¹³CH₃, Alaβ-¹³CH₃]-P1/[²H]-P2–5 CheA in the presence of various amounts of non-labeled CheY, observed at 303 K (left panel) and 283 K (right panel). Only the regions with the M3 resonances are shown. The centers of the M3 resonances are indicated with dots, and the amounts of CheY relative to CheA are indicated. (b) Overlaid resonances from the methyl group of M3 of CheA at 303 K (left panel) and 283 K (right panel), in the presence of various concentrations of CheY, simulated using the transition rates in Fig. 4a,b, with the same colors as in (a).