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Curcumin inhibited Wnt/β-catenin pathways. (A) Western blot analysis of the protein expression of β-catenin, axin, TCF4, and GAPDH in SW480 cells following curcumin treatment for 24 h. The concentrations of curcumin was 40 μM. n = 3 independent experiments and this panel presented one of these repeats. (B) RT-q-PCR of the mRNA level of Nkd2 in SW480 cells with the treatments as indicated. Data were represented as mean ± SD; n = 3 independent experiments. ∗∗∗p < 0.001.
