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. 2017 Nov 28;7:16499. doi: 10.1038/s41598-017-16486-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD44 is dispensable for the morphologic response to ECM stiffness. WT and CD44KO cells were incubated on soft and stiff FN-coated hydrogels with 10% FBS for 16 h. (A,B) Cells were fixed and imaged by phase contrast microscopy; cell area was quantified and plotted as mean ± s.e.m. for 4 independent experiments. (C) Zoom of region of interest (ROI) of phalloidin-stained images shown in Supplementary Figure S5B. (D) Average phalloidin intensity was analyzed and plotted as mean ± s.e.m. for 4 independent experiments.