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. 2017 Nov 28;7:16483. doi: 10.1038/s41598-017-16633-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of experimental design. Biofilm was cultivated in a drip flow reactor for 48 hrs and single and four-species biofilm was scraped off the glass slide. Proteins were extracted sonication, digested with trypsin and the resulting peptides were separated into three fractions using SDB-RPS filter tips. Mass spectrometric analysis was performed on a Q-Exactive and data was analyzed using MaxQuant with label free quantification.