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. 2017 Nov 28;7:16500. doi: 10.1038/s41598-017-16287-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential mRNA expression in LPMC. Genome wide expression analysis by RNAseq was performed with RNA from LPMCs isolated from rabbit caeca (n = three per group). Expression profiles of the different treatment groups were sorted by hierarchical clustering. Left) Number of under- (A) and over-expressed (B) mRNAs in the TSO DSS, Veh DSS and TSO Water groups in comparison to the control Veh Water group. Transcripts having a log2FC > |1| and P < 0.05 were considered as differentially expressed and included in further analyses. Right) Significantly affected Process Networks (PN) as identified by MetaCore™. PN without relevance to the investigated tissue and model were omitted. Numbers in parentheses provide the PN rank. (C) Validation of differential mRNA expression of COX-2, IL-6, and MMP-1 in LPMC (data pooled from two separate experiments, with n = 3–4 per group) by rt-qPCR. Expression was calculated with the ΔΔCt method relative to GAPDH and gave similar results as RNAseq (D). *P < 0.05, two-sided p-Value, Mann-Whitney test relative to vehicle water control if not indicated otherwise. Dots represent single animals, bars represent mean ± SEM.