Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 28;7:16495. doi: 10.1038/s41598-017-16472-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Functional experiments regarding the histone signature. (a) The expression levels of the 5 histone genes were determined in 4 HCC cell lines (ME180, C33A, HT3 and Caski). The histone gene expression level in the ME180 and C33A cell lines was significantly higher than that in the HT3 and Caski cell lines. (b) Micrographs showing the DNA damage marker γH2A.X in the cervical cancer cell lines mentioned above after treatment with 5 µM camptothecin for 2 hours. Non-treated images are not shown here. Cell nuclei are stained blue by Hoechst dye to indicate the cell number. The graph to the right of the micrographs shows the quantification of the proportion of cells with γH2A.X-positive nuclei among 600 cells from 6 images acquired using a 20X objective. The data were analyzed using the Analyze Particles tool in ImageJ; points larger than 30 pixels 2 were counted as a cell.