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. 2017 Nov 28;7:16503. doi: 10.1038/s41598-017-16769-5

Figure 1.

Figure 1

Generation of Sam3 gene KO mice by TALENs. (A) Schematic representation of the genomic structure of the mouse Sam3 gene. The blue character sequences indicate the positions recognized by the TALEN repeat domains. The targeting sequences are highlighted in orange. The start codon is highlighted in red. “−” denotes deleted nucleotides. (B) Gel electrophoresis of the PCR products of SAM3 genomic DNA. (C,D) RT-PCR analysis of SAM3 mRNA expression from the brains of wild-type mice and heterozygous and homozygous mutants using two different pairs of primers.