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. 2017 Nov 28;7:16503. doi: 10.1038/s41598-017-16769-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sam3 ^−/− mice had normal body weight and locomotion; however, male mice showed increased anxiety. (A) Body weight of males. Sam3 ^+/+, n = 14; Sam3 ^+/−, n = 15; Sam3 ^−/−, n = 14; one-way ANOVA with Bonferroni post hoc test, F(2,40) = 0.9056, p = 0.4124. (B) Body weight of females. Sam3 ^+/+, n = 20; Sam3 ^+/−, n = 16; Sam3 ^−/−, n = 13; one-way ANOVA with Bonferroni post hoc test, F(2,46) = 2.456, p = 0.0969. (C) Total distance traveled in the OFT by males. Sam3 ^+/+, n = 15; Sam3 ^+/−, n = 20; Sam3 ^−/−, n = 17; two-way ANOVA with Bonferroni post hoc test, F(2,245) = 1.62, p = 0.2087. (D) Total distance traveled in the OFT by females. Sam3 ^+/+, n = 19; Sam3 ^+/−, n = 19; Sam3 ^−/−, n = 13; two-way ANOVA with Bonferroni post hoc test, F(2,240) = 3.26, *p = 0.0470. (E) Percentage of time spent in the open arms in the EPM for males. Sam3 ^+/+, n = 14; Sam3 ^+/−, n = 19; Sam3 ^−/−, n = 17; one-way ANOVA with Bonferroni’s post hoc test, F(2,46) = 4.076, *p = 0.0235. (F) Percentage of time spent in the open arms in the EPM for females. Sam3 ^+/+, n = 20; Sam3 ^+/−, n = 19; Sam3 ^−/−, n = 13; one-way ANOVA with Bonferroni’s post hoc test, F(2,49) = 0.8294, p = 0.4424. Data are expressed as the mean ± SEM (*p < 0.05). (G) Representative video tracking data of male Sam3 ^+/+ mice. (H) Representative video tracking data of male Sam3 ^−/− mice.