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. 2017 Nov 28;7:16510. doi: 10.1038/s41598-017-16836-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) CE-LIFP analysis of the interaction of F-R1 and F-W1 with template and polymerase. Reaction solutions contained 1: F-R1; 2: F-R1 and template; 3: F-R1, template, and polymerase; 4: F-W1; 5: F-W1 and template; 6: F-W1, template, and polymerase. (b) CE-LIFP analysis of complex conformation in the presence of GO. Reaction solutions contained 1: F-R1, template, and polymerase; 2: F-R1, template, polymerase, and GO; 3: F-W1, template, and polymerase; 4: F-W1, template, polymerase, and GO. (c) Change of F-R1-template and F-W1-template complex concentrations in the presence of GO. The concentrations of primers (F-R1 and F-W1), template, and polymerase are 10 nM, 10 nM, and 0.5 U, and the concentration of GO is 1 μg/mL. All reaction solutions were run with a cycle of 45 s at 95 °C, 1 min at 50 °C, and 1 min extension at 72 °C before CE-LIFP analysis.