Table 1.
Fatty acid composition (as percentage of total fatty acids) of ten British thraustochytrid isolates and reference strain Sicyoidochytrium sp. (NBRC 102979) grown in MCBHB medium in shake flask culture and harvested at the end of the exponential phase and late stationary phase. Values represent mean ± standard deviation (n = 3)
| Strain | SW4T2A | SW4T2B | SW4T8A | OL5TA | SW7T4C1 | SW7T4C6 | SW7T7C | SW7T8C | TL18 | SM01 | 102979 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Growth stage | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat |
| Fatty acids | ||||||||||||||||||||||
| 14:0 | 2.5 ± 0.4 | 1.1 ± 0.2 | 1.9 ± 1.1 | 0.7 ± 0.1 | 0.8 ± 0.3 | 0.7 ± 0.1 | 1 ± 0.3 | 0.5 ± 0 | 2.3 ± 0.1 | 1.4 ± 0.2 | 2.8 ± 0.5 | 2.1 ± 0.2 | 3.2 ± 1.6 | 1.2 ± 0.6 | 5.7 ± 0.6 | 5.2 ± 0.5 | 5.6 ± 5 | 1.7 ± 0.2 | 0.4 ± 0 | 0.4 ± 0.3 | 0.9 ± 0.1 | 0.5 ± 0.1 |
| 15:0 | 14.7 ± 3.6 | 10 ± 1.2 | 5.7 ± 3.8 | 6.6 ± 1.4 | 16.1 ± 1.1 | 10.3 ± 0.4 | 11.3 ± 2.4 | 5.6 ± 0.1 | 9.7 ± 0.6 | 5 ± 0.8 | 14.4 ± 2.3 | 9.4 ± 0.5 | 17.3 ± 3.5 | 7.6 ± 1.7 | 19.8 ± 0.4 | 18.1 ± 3.9 | 9.6 ± 8 | 6.2 ± 0.4 | 10.2 ± 1.4 | 8.6 ± 1.9 | 4 ± 0.7 | 2.2 ± 0.1 |
| 16:0 | 6.5 ± 1.3 | 8.7 ± 1.5 | 13.2 ± 11.5 | 9.1 ± 1.8 | 5.4 ± 0.5 | 4.8 ± 0.2 | 5.4 ± 1.1 | 3.8 ± 0.4 | 16.5 ± 1.2 | 15.6 ± 1.1 | 12.3 ± 2.4 | 13.6 ± 0.8 | 12 ± 2 | 9.7 ± 2.3 | 9.4 ± 0.2 | 12.8 ± 2.3 | 21.1 ± 13.8 | 8.5 ± 0.2 | 5.9 ± 0.7 | 6.2 ± 1 | 37.3 ± 0.6 | 27.8 ± 1.4 |
| 17:0 | 4.2 ± 1.2 | 5 ± 0.5 | 9.5 ± 9.2 | 6.3 ± 1.3 | 5.4 ± 0.6 | 4.4 ± 0.2 | 4 ± 0.8 | 3.3 ± 0.1 | 6 ± 0.4 | 3.6 ± 0.4 | 6.3 ± 1.2 | 4.8 ± 0.2 | 5.5 ± 0.7 | 4.3 ± 0.2 | 3.2 ± 0.1 | 4.5 ± 1 | 8.2 ± 5.3 | 3.7 ± 0.3 | 9.8 ± 1.5 | 8.5 ± 1.4 | 1.9 ± 0.2 | 1.1 ± 0 |
| 18:1ω9c | 0.9 ± 0.1 | 0.5 ± 0.1 | 1.1 ± 0.6 | 0.4 ± 0 | 0.9 ± 0.1 | 0.6 ± 0 | 1.6 ± 0.2 | 0.7 ± 0 | 0.5 ± 0.1 | 0.3 ± 0 | 0.7 ± 0.1 | 0.6 ± 0 | 1.6 ± 0.4 | 0.8 ± 0.3 | 1.1 ± 0 | 1.2 ± 0.2 | 1.2 ± 0.7 | 0.5 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.8 ± 0.3 | 0.2 ± 0 |
| 18:1ω7c | 1.7 ± 0.3 | 2.1 ± 0.4 | 4.7 ± 3.9 | 3.5 ± 0.7 | 1.5 ± 0.1 | 1.3 ± 0.1 | 4.9 ± 0.7 | 3.2 ± 0.3 | 0.6 ± 0 | 0.7 ± 0 | 0.6 ± 0.1 | 0.6 ± 0 | 1.5 ± 0.2 | 1.8 ± 0.8 | 0.8 ± 0 | 1.3 ± 0.2 | 1.4 ± 0.8 | 0.8 ± 0 | 1.8 ± 0.2 | 1.3 ± 0.3 | 2.6 ± 0.5 | 4.5 ± 0.1 |
| 20:4ω6 ARA | 1.1 ± 0.1 | 1 ± 0 | 0.6 ± 0.4 | 0.6 ± 0.1 | 1.1 ± 0 | 1.1 ± 0 | 1.4 ± 0.1 | 1 ± 0 | 0.5 ± 0.1 | 0.6 ± 0 | 1.6 ± 0.1 | 1.4 ± 0 | 1.3 ± 0.2 | 1.2 ± 0.3 | 1.4 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.4 | 1.1 ± 0 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0 | 0.4 ± 0 |
| 20:5ω3 EPA | 1.4 ± 0.2 | 3.1 ± 0.2 | 2 ± 1.3 | 4 ± 0.2 | 3.2 ± 0 | 4.3 ± 0.2 | 2.9 ± 0.3 | 4.5 ± 0.1 | 2.5 ± 0.2 | 4.3 ± 0.3 | 3 ± 0.4 | 4.5 ± 0.2 | 2.7 ± 0.8 | 4.7 ± 0.5 | 2.1 ± 0.2 | 3.1 ± 0.6 | 3.3 ± 1.3 | 5.2 ± 0.3 | 2.4 ± 0.1 | 3.4 ± 0.2 | 2.1 ± 0.2 | 2.4 ± 0.1 |
| 22:5ω6 DPAω6 | 3.3 ± 0.1 | 4 ± 0 | 3.3 ± 1.3 | 4.4 ± 0.4 | 1.1 ± 0.2 | 1.7 ± 0.1 | 1.3 ± 0.3 | 1 ± 0.3 | 5.8 ± 0.1 | 7 ± 0.3 | 5.7 ± 0.5 | 6.7 ± 0.2 | 1.8 ± 0.3 | 1.4 ± 0 | 4.1 ± 0.1 | 4.2 ± 0.5 | 4 ± 1.6 | 6.2 ± 0.2 | 4.4 ± 0.1 | 4.8 ± 0.2 | 7.6 ± 0.4 | 1.5 ± 0.6 |
| 22:6ω3 DHA | 46.8 ± 6.7 | 52 ± 3.8 | 47.3 ± 18.2 | 55 ± 4.3 | 51.2 ± 2.8 | 58.5 ± 1 | 48.5 ± 6.2 | 63.2 ± 1.7 | 47.9 ± 1.6 | 54.5 ± 2.9 | 41.4 ± 6.4 | 47.1 ± 1 | 38.1 ± 7.9 | 54.3 ± 4.7 | 38.1 ± 0.9 | 37.2 ± 6.2 | 36.1 ± 16.6 | 60.7 ± 0.3 | 55.3 ± 3.7 | 57.4 ± 5 | 30 ± 1.9 | 52.1 ± 2.3 |
| 22:4ω6 DTA | 0.4 ± 0 | 0.2 ± 0.3 | 0.5 ± 0.3 | 0.9 ± 0.2 | 0.6 ± 0.1 | 0.8 ± 0 | 0.9 ± 0 | 0.8 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0 | 0.6 ± 0.7 | 0.8 ± 0.1 | 0.5 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0.3 | 0.5 ± 0.5 | 0.9 ± 0.7 | 0 ± 0 | 0.9 ± 0.1 | 0.8 ± 0.7 | 0.4 ± 0 | 0.4 ± 0 |
| 22:5ω3 DPAω3 | 1.2 ± 0.2 | 1.8 ± 0.3 | 1.1 ± 0.8 | 2.5 ± 0.3 | 1.1 ± 0.1 | 1 ± 0.1 | 0.9 ± 0.1 | 1.4 ± 0.1 | 0.9 ± 0.6 | 1.6 ± 0.1 | 0.9 ± 0.6 | 1 ± 0.6 | 0.9 ± 0.1 | 1.5 ± 0.1 | 1.2 ± 0.4 | 1.7 ± 0.1 | 1.6 ± 0.7 | 2 ± 0.1 | 1.1 ± 0.1 | 1.5 ± 1.6 | 0.8 ± 0 | 1.0 ± 0 |
| Sum SFA | 27.9 ± 4 | 24.7 ± 1.9 | 30.3 ± 15.2 | 22.7 ± 2.7 | 27.7 ± 1.4 | 20.2 ± 0.5 | 21.7 ± 2.8 | 13.2 ± 0.4 | 34.5 ± 1.4 | 25.7 ± 1.4 | 35.7 ± 3.6 | 29.9 ± 0.9 | 38 ± 4.4 | 22.8 ± 3 | 38.2 ± 0.8 | 40.6 ± 4.7 | 44.6 ± 17.5 | 20.1 ± 0.5 | 26.3 ± 2.1 | 23.7 ± 2.6 | 44.1 ± 0.9 | 31.6 ± 1.4 |
| Sum MUFA | 2.6 ± 0.3 | 2.6 ± 0.4 | 5.8 ± 4 | 3.8 ± 0.7 | 2.4 ± 0.2 | 1.9 ± 0.1 | 6.5 ± 0.7 | 3.9 ± 0.3 | 1 ± 0.1 | 1 ± 0.1 | 1.3 ± 0.2 | 1.2 ± 0.1 | 3 ± 0.4 | 2.6 ± 0.9 | 1.8 ± 0.1 | 2.5 ± 0.3 | 2.6 ± 1 | 1.3 ± 0.1 | 2.1 ± 0.2 | 1.6 ± 0.3 | 3.4 ± 0.6 | 4.8 ± 0.1 |
| Sum PUFA | 54.3 ± 6.7 | 62 ± 3.8 | 54.7 ± 18.3 | 67.5 ± 4.3 | 58.4 ± 2.8 | 67.4 ± 1 | 55.9 ± 6.2 | 71.8 ± 1.7 | 58.3 ± 1.7 | 68.6 ± 2.9 | 53.3 ± 6.5 | 61.4 ± 1.2 | 45.4 ± 7.9 | 64 ± 4.7 | 47.3 ± 1 | 47.9 ± 6.2 | 47.2 ± 16.8 | 75.1 ± 0.5 | 64.9 ± 3.7 | 68.6 ± 5.4 | 41.3 ± 2 | 57.9 ± 2.4 |
| Sum ω3 | 49.4 ± 6.7 | 56.8 ± 3.8 | 50.4 ± 18.2 | 61.5 ± 4.3 | 55.5 ± 2.8 | 63.8 ± 1 | 52.3 ± 6.2 | 69 ± 1.7 | 51.3 ± 1.7 | 60.3 ± 2.9 | 45.3 ± 6.4 | 52.5 ± 1.2 | 41.7 ± 7.9 | 60.6 ± 4.7 | 41.5 ± 1 | 42 ± 6.2 | 41 ± 16.7 | 67.8 ± 0.4 | 58.8 ± 3.7 | 62.3 ± 5.3 | 32.9 ± 1.9 | 55.5 ± 2.3 |
| Sum ω6 | 4.9 ± 0.1 | 5.2 ± 0.3 | 4.3 ± 1.3 | 5.9 ± 0.4 | 2.9 ± 0.2 | 3.6 ± 0.1 | 3.6 ± 0.3 | 2.8 ± 0.3 | 7 ± 0.1 | 8.3 ± 0.3 | 7.9 ± 0.9 | 8.9 ± 0.2 | 3.7 ± 0.3 | 3.4 ± 0.1 | 5.9 ± 0.3 | 5.9 ± 0.7 | 6.2 ± 1.7 | 7.3 ± 0.2 | 6.1 ± 0.1 | 6.3 ± 0.7 | 8.3 ± 0.4 | 2.3 ± 0.6 |
ARA arachidonic acid, EPA eicosapentaenoic acid, DPAω6 docosapentaenoic acid (also termed Osbond acid), DHA docosahexaenoic acid, DTA docosatetraenoic acid, DPAω3 docosapentaenoic acid (also termed Clupanodonic acid), Exp end of exponential phase, Stat late stationary phase