Table 2.
Fatty acid composition (as percentage of total fatty acids) of ten British thraustochytrid isolates and reference strain Sicyoidochytrium sp. (NBRC 102979) grown in modified GYP medium in shake flask culture and harvested at the end of the exponential phase and late stationary phase. Values represent mean ± standard deviation (n = 3)
| Strains | SW4T2A | SW4T2B | SW4T8A | OL5TA | SW7T4C1 | SW7T4C6 | SW7T7C | SW7T8C | TL18 | SM01 | 102979 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Growth stage | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat | Exp | Stat |
| Fatty acids | ||||||||||||||||||||||
| 14:0 | 3.3 ± 0.1 | 1.9 ± 0.1 | 1.6 ± 0.7 | 1.2 ± 0.2 | 1.7 ± 0.3 | 1.2 ± 0 | 0.7 ± 0.1 | 0.6 ± 0 | 1.3 ± 0.3 | 0.6 ± 0.1 | 2.2 ± 0.6 | 1.2 ± 0.1 | 1.6 ± 0.2 | 0.6 ± 0.2 | 0.6 ± 0.1 | 0.5 ± 0 | 0.5 ± 0.2 | 0.4 ± 0 | 0.5 ± 0.1 | 0.6 ± 0.1 | 1.4 ± 0 | 0.8 ± 0.1 |
| 15:0 | 17.7 ± 1.3 | 11.8 ± 0.2 | 13.7 ± 5.4 | 1.4 ± 3.1 | 11.4 ± 0.7 | 8.5 ± 0.1 | 9 ± 1.6 | 6.4 ± 0.3 | 10.1 ± 2.3 | 5.9 ± 0.3 | 8.4 ± 5.9 | 2.2 ± 3.7 | 18.9 ± 3.9 | 6.9 ± 1.2 | 6 ± 0.5 | 5.7 ± 0.5 | 4.3 ± 3.8 | 3.8 ± 3.3 | 4.3 ± 3.7 | 6.3 ± 0.2 | 2.2 ± 0.2 | 1.7 ± 0 |
| 16:0 | 15.2 ± 0.6 | 11.9 ± 0.5 | 10.7 ± 2.4 | 10.2 ± 2.2 | 8.6 ± 0.8 | 7.6 ± 0.1 | 8.1 ± 0.8 | 7 ± 0.8 | 7.1 ± 1.9 | 4.4 ± 0.6 | 14.1 ± 3.4 | 10.6 ± 0.9 | 9.2 ± 0.3 | 6.5 ± 0.7 | 4.7 ± 0.8 | 4.8 ± 0.2 | 5.4 ± 0.5 | 5.6 ± 0.4 | 4.1 ± 0.1 | 4.1 ± 0.1 | 58.4 ± 2.4 | 27.4 ± 0.6 |
| 17:0 | 5.8 ± 0.4 | 4.4 ± 0.2 | 6.4 ± 1.8 | 4.8 ± 0.9 | 3.2 ± 0.2 | 2.9 ± 0.1 | 4.3 ± 0.5 | 2.9 ± 0.1 | 4.2 ± 1.1 | 2.7 ± 0.2 | 5.8 ± 1.6 | 3.4 ± 0.2 | 9 ± 1.5 | 4.4 ± 0.6 | 2.6 ± 0.3 | 2.8 ± 0.1 | 3.5 ± 0.1 | 3.4 ± 0.1 | 2.7 ± 0.1 | 2.6 ± 0.1 | 0.7 ± 0 | 0.3 ± 0 |
| 18:1ω9c | 1 ± 0 | 0.6 ± 0 | 1 ± 0.4 | 0.6 ± 0.2 | 0.7 ± 0.1 | 0.5 ± 0 | 0.5 ± 0.1 | 0.4 ± 0 | 0.6 ± 0.1 | 0.2 ± 0 | 1.4 ± 0.4 | 0.8 ± 0.1 | 1 ± 0.1 | 0.5 ± 0.1 | 0.3 ± 0 | 0.2 ± 0 | 0.3 ± 0 | 0.3 ± 0 | 0.3 ± 0 | 0.3 ± 0 | 2.7 ± 1.9 | 0.1 ± 0 |
| 18:1ω7c | 4 ± 0.1 | 2.6 ± 0.2 | 4.2 ± 0.8 | 2.2 ± 1.2 | 1.4 ± 0.2 | 0.8 ± 0.1 | 1.1 ± 0.1 | 0.8 ± 0.2 | 1.7 ± 0.4 | 0.4 ± 0.1 | 4.3 ± 2.9 | 4.6 ± 0.4 | 2.2 ± 0.1 | 1.4 ± 0.1 | 0.7 ± 0.1 | 0.8 ± 0.1 | 0.7 ± 0 | 0.7 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.8 ± 0 | 2.6 ± 0.1 |
| 20:4ω6 ARA | 0.8 ± 0 | 0.9 ± 0.1 | 1 ± 0.1 | 1.1 ± 0 | 0.9 ± 0 | 0.9 ± 0 | 0.8 ± 0.1 | 0.7 ± 0.1 | 1.1 ± 0.1 | 0.9 ± 0.1 | 1.1 ± 0.1 | 1.2 ± 0 | 0.8 ± 0 | 0.8 ± 0 | 1 ± 0.1 | 0.8 ± 0 | 0.9 ± 0 | 0.7 ± 0.1 | 1.1 ± 0 | 1 ± 0 | 0.2 ± 0 | 0.5 ± 0 |
| 20:5ω3 EPA | 2.4 ± 0.1 | 3.4 ± 0.1 | 3.7 ± 1.3 | 5.4 ± 0.3 | 4.1 ± 0.2 | 4.9 ± 0.1 | 4.2 ± 0.7 | 4.2 ± 0.7 | 5.1 ± 0.5 | 7.5 ± 0.5 | 3 ± 0.5 | 4.5 ± 0.3 | 2.8 ± 0.4 | 4.7 ± 0.2 | 4.9 ± 0 | 5.1 ± 0.1 | 4.9 ± 0.1 | 4.9 ± 0.5 | 4.3 ± 0.1 | 4.9 ± 0.2 | 1 ± 0.1 | 2.4 ± 0 |
| 22:5ω6 DPAω6 | 2 ± 0.1 | 2.4 ± 0.2 | 2.5 ± 0.5 | 3.5 ± 0.2 | 3.5 ± 0.4 | 3.4 ± 0 | 2.4 ± 0.2 | 1.8 ± 0.4 | 3.5 ± 0.2 | 2.9 ± 0.7 | 2.3 ± 0.3 | 3.1 ± 0.4 | 2.4 ± 0.1 | 2.3 ± 0.5 | 1.5 ± 0.2 | 1.6 ± 0.2 | 1.5 ± 0.5 | 1.4 ± 0.4 | 1.4 ± 0.1 | 2.1 ± 0.5 | 4.1 ± 0.1 | 6.2 ± 1.1 |
| 22:6ω3 DHA | 26.4 ± 2 | 39.8 ± 1.5 | 31.7 ± 9.9 | 46 ± 2.6 | 45.9 ± 2.6 | 52.4 ± 0.6 | 51.9 ± 3.6 | 60 ± 1.3 | 45.6 ± 7.4 | 58.9 ± 1.9 | 30.3 ± 7.1 | 44.8 ± 0.3 | 35.9 ± 5.9 | 57.1 ± 3.8 | 65.5 ± 0.5 | 65.9 ± 0.3 | 66.4 ± 2.3 | 66.9 ± 2 | 60.7 ± 2.1 | 58.2 ± 0.3 | 11.8 ± 0.4 | 51.6 ± 1.5 |
| 22:4ω6 DTA | 0.5 ± 0 | 0.6 ± 0.1 | 0.7 ± 0.1 | 1 ± 0.1 | 0.9 ± 0 | 0.9 ± 0 | 0.7 ± 0.1 | 0.7 ± 0.1 | 0.8 ± 0.1 | 1 ± 0.1 | 0.7 ± 0.1 | 0.8 ± 0.1 | 0.6 ± 0 | 0.8 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0 | 0.7 ± 0.1 | 0.7 ± 0 | 0.5 ± 0 | 0.5 ± 0.1 | 0.2 ± 0 | 0.7 ± 0.1 |
| 22:5ω3 DPAω3 | 1.2 ± 0.1 | 1.7 ± 0.2 | 2.5 ± 0.9 | 4.1 ± 0.2 | 1.4 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.4 | 1.8 ± 0.2 | 1.8 ± 0.2 | 2.6 ± 0.1 | 0.9 ± 0.1 | 1.4 ± 0.2 | 1.5 ± 0 | 2.2 ± 0 | 1.8 ± 0.1 | 2.1 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.1 | 2 ± 0.3 | 2.2 ± 0.5 | 0.4 ± 0.1 | 1.2 ± 0.1 |
| Sum SFA | 42.1 ± 1.5 | 30 ± 0.6 | 32.4 ± 6.2 | 17.6 ± 3.9 | 25 ± 1.1 | 20.2 ± 0.2 | 22.1 ± 1.9 | 16.9 ± 0.8 | 22.7 ± 3.2 | 13.6 ± 0.6 | 30.4 ± 7 | 17.3 ± 3.8 | 38.6 ± 4.2 | 18.5 ± 1.5 | 13.9 ± 1.1 | 13.8 ± 0.5 | 13.7 ± 3.8 | 13.2 ± 3.4 | 11.6 ± 3.7 | 13.6 ± 0.3 | 62.7 ± 2.5 | 30.3 ± 0.6 |
| Sum MUFA | 5 ± 0.1 | 3.3 ± 0.2 | 5.1 ± 0.9 | 2.8 ± 1.3 | 2 ± 0.3 | 1.3 ± 0.1 | 1.6 ± 0.1 | 1.2 ± 0.2 | 2.3 ± 0.4 | 0.7 ± 0.1 | 5.7 ± 2.9 | 5.4 ± 0.4 | 3.2 ± 0.2 | 1.9 ± 0.1 | 1 ± 0.1 | 1 ± 0.1 | 1 ± 0 | 1 ± 0.1 | 1.5 ± 0.1 | 1.5 ± 0.1 | 4.5 ± 1.9 | 2.8 ± 0.1 |
| Sum PUFA | 33.2 ± 2 | 48.8 ± 1.5 | 42.2 ± 10 | 61.1 ± 2.7 | 56.8 ± 2.7 | 64.2 ± 0.6 | 61.8 ± 3.7 | 69.1 ± 1.6 | 58 ± 7.4 | 73.8 ± 2.1 | 38.3 ± 7.1 | 55.8 ± 0.6 | 44 ± 5.9 | 67.8 ± 3.8 | 75.4 ± 0.6 | 76.3 ± 0.4 | 76 ± 2.4 | 76.5 ± 2.1 | 70.1 ± 2.2 | 69 ± 0.8 | 17.7 ± 0.4 | 62.5 ± 1.9 |
| Sum ω3 | 30 ± 2 | 44.9 ± 1.5 | 38 ± 10 | 55.5 ± 2.7 | 51.4 ± 2.7 | 59 ± 0.6 | 57.8 ± 3.7 | 65.9 ± 1.5 | 52.6 ± 7.4 | 69 ± 1.9 | 34.2 ± 7.1 | 50.7 ± 0.5 | 40.2 ± 5.9 | 64 ± 3.8 | 72.3 ± 0.6 | 73.1 ± 0.3 | 73 ± 2.3 | 73.6 ± 2 | 67 ± 2.2 | 65.3 ± 0.6 | 13.2 ± 0.4 | 55.1 ± 1.5 |
| Sum ω6 | 3.2 ± 0.1 | 3.9 ± 0.2 | 4.2 ± 0.5 | 5.6 ± 0.2 | 5.4 ± 0.4 | 5.2 ± 0 | 4 ± 0.2 | 3.2 ± 0.4 | 5.5 ± 0.2 | 4.8 ± 0.7 | 4.1 ± 0.3 | 5.1 ± 0.4 | 3.7 ± 0.1 | 3.8 ± 0.5 | 3.1 ± 0.2 | 3.2 ± 0.2 | 3 ± 0.5 | 2.9 ± 0.4 | 3 ± 0.1 | 3.7 ± 0.5 | 4.5 ± 0.1 | 7.4 ± 1.1 |
Abbreviations: see Table 1