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. 2017 Nov 29;37(6):BSR20171092. doi: 10.1042/BSR20171092

Figure 3. Wnt5a induces EMT in NSCLC cells.

Figure 3

(A) The effects of siRNA-mediated silencing of Wnt5a on the expression of N-cadherin, vimentin, and E-cadherin in A549 cells by the Western blotting analysis. (B) Luciferase reporter assays using TopFlash/FopFlash reporter plasmids to monitor the activity of Tcf/Lef transcription factor. (C) The effects of Wnt5a knockdown on the expression level of nuclear β-catenin. Lamin A/C was used as a nuclear internal control by the Western blotting analysis. (D) The effects of Wnt5a knockdown on the expression levels of ROR2 and p-ROR2 in A549 cells by the Western blotting analysis. (E) The effects of Wnt5a overexpression on the expression of N-cadherin, vimentin, and E-cadherin in A549 cells by the Western blotting analysis. (F) Luciferase reporter assays using TopFlash/FopFlash reporter plasmids to monitor the activity of Tcf/Lef transcription factor. (G) The effects of Wnt5a overexpression on the expression level of nuclear β-catenin. Lamin A/C was used as a nuclear internal control by the Western blotting analysis. (H) The effects of Wnt5a overexpression on the expression levels of ROR2 and p-ROR2 in A549 cells by the Western blotting analysis. A549 cells that transfected with the empty expression vector pcDNA6.2 and H1975 cells that transfected with the empty expression vector pcDNA3.1 were the corresponding controls.