FIG 6 .

CD226 is not required for LCL proliferation or sensitivity to apoptosis relative to controls. (A) Growth of the WT LCL, sgAAVS1, and sgCD226-1 and -2 LCLs seeded at 200,000 cells/ml from three independent experiments. Data are shown as means ± SD. (B) The WT LCL, sgAAVS1, and sgCD226-1 and -2 LCLs were treated with Nutlin-3 (10 μM [two independent experiments]) or camptothecin (CPT; 15 μM [three independent experiments]) for 24 h. Apoptosis was quantified by flow cytometry for annexin V positivity. Data are reported as means ± SD. (C) Relative binding of WT, sgAAVS1, and sgCD226-1 and -2 LCLs to activated HUVECs from two independent experiments performed in triplicate. Data are reported as means ± SD. (D) Bright-field images of WT, sgAAVS1, and sgCD226-1 and -2 LCLs during culture (magnification, 40×).