FIG 2.

Effect of mutant p53 proteins on mitochondrial metabolism. (A to C) Measurement of mitochondrial oxygen consumption rates (OCRs) in stable H1299 cell lines (A) and in tetracycline (Tet)- or doxycycline (Dox)-induced expression of p53R175H and p53R273H mutants in HCT116 or H1299 cells, respectively (B and C). OCR values of maximal respiration and ATP generated per consumed oxygen level were calculated. (D) Western blot of p53 in H1299 control (CV) cells with transient expression of the indicated mutant p53 proteins. β-Actin was used as a loading control. (E) Mutant p53 expression was induced as described for panel D, and mitochondrial OCRs were analyzed. Data are expressed as means ± SD (n = 3). Statistical significance is shown above the level for the control. *, P < 0.05; **, P < 0.001; ***, P < 0.0001; two-tailed Student's t test.