TABLE 2.
Antibodies used in this study
| Antibody | Purpose | Dilution or amt |
|---|---|---|
| Antinucleolin (ab13541) | IFA | 1:300 |
| Antinucleolin (ab22758) | IFA | 1:500 |
| Western blotting | 1:2,000 | |
| IP | 2 μg | |
| Antinucleolin (ab50279) | Immuno-RNA-FISH | 1:500 |
| Anti-lamin A/C (Epitomics, 2966S) | IFA | 1:600 |
| Western blotting | 1:5,000 | |
| Anti-lamin A/C (ab40567) | IFA | 1:50 |
| Western blotting | 1:200 | |
| Anti-lamin B2 (ab8983) | IFA | 1:400 |
| Western blotting | 1:400 | |
| Antifibrillarin (ab5821) | IFA | 1:500 |
| Antinucleophosmin (ab37659) | Western blotting | 1:1,000 |
| IP | 2 μg | |
| Anti-p84 (ab487) | IFA | 1:500 |
| Antinucleophosmin (ab10530) | Western blotting | 1:500 |
| Antiactin | Western blotting | 1:400 |
| Anti-GAPDH | Western blotting | 1:5,000 |
| Anti-rabbit antibody–Alexa Fluor 488 | IFA | 1:1,000 |
| Anti-rabbit antibody–Alexa Fluor 568 | IFA | 1:1,000 |
| Anti-mouse antibody–Alexa Fluor 488 | IFA | 1:1,000 |
| Anti-mouse antibody–Alexa Fluor 568 | IFA | 1:1,000 |
| Anti-mouse antibody–Alexa Fluor 633 | IFA | 1:1,000 |
| Donkey anti-rabbit antibody–horseradish peroxidase (GE, NA9340V) | Western blotting | 1:10,000 |
| Sheep anti-mouse antibody–horseradish peroxidase (GE, NA9310V) | Western blotting | 1:10,000 |
| Normal rabbit IgG | IP | 2 μg |