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. 2017 Nov 28;37(24):e00337-17. doi: 10.1128/MCB.00337-17

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Copyright © 2017 Ishimoto et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Immunohistochemical analysis of PGC-1α and 8-OHdG in the kidneys of ADPKD model animals. (A) Representative IHC staining for PGC-1α in kidney tissue from 7-day-old Ksp-Cre PKD1^flox/+ and Ksp-Cre PKD1^flox/flox mice. Left, cortex of PKD1^flox/+ mouse kidney; middle and right, lower and higher magnifications of PKD1^flox/flox mouse kidney, respectively. The area surrounded by the dotted line in the higher-magnification image represents cyst-lining cells. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (B) Representative IHC staining for PGC-1α in kidney tissue from 7-week-old Cy rats. Left, IHC results for controls (+/+); right, IHC results for Cy/+ rats. The bar graph shows the relative ratio of staining intensity of diaminobenzidine (DAB) in normal tubules and cyst-lining cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (C) Representative IHC staining results for 8-OHdG in kidney tissue from 7-day-old Ksp-Cre PKD1^flox/flox mice. Left, kidney tissue from Ksp-Cre PKD1^flox/+ mice; middle and right, kidney tissue from Ksp-Cre PKD1^flox/flox mice and high magnification of the black box in the middle panel, respectively. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Ksp-Cre PKD1^flox/flox mice. More than 50 cells were analyzed for staining intensity of DAB in both groups. *, representative cyst. (D) Representative IHC staining results for 8-OHdG in kidney tissue from 7-week-old Cy rats. *, representative cyst. The bar graph shows the relative ratio of staining intensity compared with that of normal tubules in Cy rats. The 8-OHdG staining intensity was increased in cyst-lining cells compared with normal tubular cells. More than 50 cells were analyzed for staining intensity of DAB in both groups. Results are shown as the relative ratio. Results represent the means ± standard deviations. **, P < 0.01; ***, P < 0.001.