Figure 1.

3D cell-based high-throughput screening chips. (a) Photo and schematic view of micropillar and microwell chip platform. Green dots are 3D-cultured astrocytes and glioblastoma multiforme (GBM) cells in alginate spot on the micropillar. (b) Schematic view of the experimental procedure. Cells are dispensed and immobilized in alginate onto the top of the micropillars and dipped in the microwells containing growth media for 1-day culture by sandwiching the micropillar and microwell chips. Compounds are dispensed into the microwells and cells are exposed to the compounds by moving the micropillar chip to a new microwell chip. 3D-cultured cells are stained with Calcein AM, and the dried alginate spot on the micropillar chip is scanned for data analysis.