FIG 1 .
Branched pathway for tetrahydrofolate and tetrahydromonapterin synthesis in B. pseudomallei (modified from Fig. 1 in the work of Podnecky et al. [1]). Blue text in scheme ii represents native metabolites that are expected to decrease in abundance relative to scheme i following treatment with SMX. Red text in scheme iii represents native metabolites that are expected to increase in abundance relative to scheme i due to folM mutation. The asterisk indicates an unmetabolizable product of SMX metabolism. Abbreviations: H2-NPt, 7,8-dihydroneopterin; H2-MPt, 7,8-dihydromonapterin; H4-MPt, tetrahydromonapterin; H2-HMPt-P2, 6-hydroxymethyl-7,8-dihydropterin pyrophosphate; PABA, para-aminobenzoic acid; H2-Pte, dihydropteroate; H2-PteGlu, dihydrofolate; H4-PteGlu, tetrahydrofolate; SMX, sulfamethoxazole.