Table 4.
Effects of GSPE on antioxidant parameters in the liver of broilers fed diets contaminated with AFB1.
| Parameters | Dietary Treatments | ||||
|---|---|---|---|---|---|
| Control | AFB1 | GSPE | AFB1 + GSPE | AFB1 + GSPE | |
| (1 mg/kg) | (250 mg/kg) | (250 mg/kg) | (500 mg/kg) | ||
| MDA, nmol/mgprot | 0.96 ± 0.05 c,d | 1.59 ± 0.17 a | 0.82 ± 0.10 d | 1.09 ± 0.08 b,c | 1.14 ± 0.13 b |
| T-SOD, U/mgprot | 71.87 ± 4.59 a | 52.70 ± 7.04 b | 79.19 ± 2.75 a | 76.14 ± 6.67 a | 76.52 ± 8.50 a |
| GSH-Px, U/mgprot | 51.65 ± 4.66 b | 29.77 ± 3.56 d | 62.46 ± 5.94 a | 40.29 ± 3.54 c | 43.81 ± 4.98 c |
| CAT, U/mgprot | 86.79 ± 9.14 a | 57.47 ± 9.12 c | 95.46 ± 8.85 a | 75.22 ± 5.16 b | 73.34 ± 6.42 b |
| GSH, mg/gprot | 2.83 ± 0.18 b | 2.09 ± 0.20 c | 3.10 ± 0.22 a | 2.78 ± 0.18 b | 2.71 ± 0.23 b |
| GR, U/gprot | 6.65 ± 0.65 a | 3.18 ± 0.30 c | 7.03 ± 0.48 a | 4.04 ± 0.42 b | 3.68 ± 0.34 b,c |
| GST, U/mgprot | 25.28 ± 1.44 a | 19.03 ± 1.48 c | 26.49 ± 2.25 a | 21.96 ± 1.46 b | 22.82 ± 1.77 b |
Values are represented as the mean ± SD (n = 6). a–d Mean values within a row with different superscript letters were significantly different (p < 0.05). AFB1, aflatoxin B1; GSPE, grape seed proanthocyanidin extract; MDA, malondialdehyde; T-SOD, total superoxide dismutase; GSH-Px, glutathione peroxidase; CAT, catalase; GSH, glutathione; GR, glutathione reductase; GST, glutathione S-transferase.