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. 2017 Oct 23;8(19):3933–3938. doi: 10.7150/jca.20342

Figure 1.

Figure 1

Hypoxia-induced changes in expression of FAM13A, IREB2 and HIF1α in lung cancer cell lines and human lung fibroblasts. A. Quantitative analysis of FAM13A, IREB2 and HIF1α mRNA expression in 3 cell lines. Cells were cultured in hypoxia or ambient oxygen (normoxia) for 72 hours. Gene expression was normalized to the endogenous control, GUSB. Mean normalized expression levels in hypoxia and normoxia are shown. Data are expressed as the mean ± SEM of triplicate values. Results are mean from three independent experiments. A549, human non-small cell lung carcinoma cell line; CORL-105, Caucasian lung adenocarcinoma cell line; HL, human lung fibroblast; N, normoxia (blue); H, hypoxia (red). *P < 0.001, **P < 0.0001. B. Western blot analysis of FAM13A, IREB2 and β-actin proteins expression in 3 cell lines, from two separate experiments. Cells were cultured in hypoxia or ambient oxygen (normoxia) for 72 hours and total proteins were extracted. A549, human non-small cell lung carcinoma cell line; CORL-105, Caucasian lung adenocarcinoma cell line; HL, human lung fibroblast; Hypoxia exposition +; Normoxia, -.