Figure 1.
Huaier inhibited proliferation and induced apoptosis of HCC cells. A. Cell viabilities were measured by CCK-8 in L-02, Bel-7404, Bel-7402 and SMMC-7721 cells treated with Huaier at the concentrations of 0, 5, 10 ,15 mg/ml for 24h. Data was represented with GraphPad Prism 5.02. *p<0.05, **p<0.01 compared with control group. B. Colony formation was performed to test the effects of Huaier (0 and 15mg/ml) on L-02, Bel-7404, Bel-7402 and SMMC-7721 cells. The colonies were then stained with 0.1% crystal violet after 7 days. C. The protein expressions of Ki-67, CyclinD1 and PCNA in Bel-7404, Bel-7402 and SMMC-7721 cells treated with Huaier at the concentrations of 0, 5, 10 ,15 mg/ml for 24h. D. The protein expressions of Bcl-2 and Bax in Bel-7404 cells treated with Huaier at the concentrations of 0, 5, 10 ,15 mg/ml for 24h. E. The protein expressions of cleaved caspase substrates in Bel-7404 and SMMC-7721 cells treated with Huaier at the concentrations of 0, 5, 10 and 15 mg/ml for 24h.