Figure 2.
Huaier inhibited migration of HCC cells. A. Wound healing assay was performed to test the effects of Huaier (15mg/ml) on Bel-7404 and SMMC-7721 cells. Selecting the same point of view to take a picture at 0h, 12h and 24h, and the relative scratch area was measured by ImageJ 1.74v. Data was represented with GraphPad Prism 5.02. *p<0.05, **p<0.01 compared with control group. B. Transwell assay was performed to test the effects of Huaier (0 and 15mg/ml, for 24h) on migration of Bel-7404 and SMMC-7721 cells. Cells on the lower surface of transwell membrane were stained with 0.1% crystal violet. Data was represented with GraphPad Prism 5.02. ***p<0.001 compared with control group. C. The protein expressions of E-cadherin and N-cadherin in L-02, Bel-7404, Bel-7402 and SMMC-7721 cells treated with Huaier at the concentrations of 0, 5, 10 and 15 mg/ml for 24h.