Figure 3.
Overexpression of YAP1 induced HCC cell proliferation and migration. A. The tumor-related mRNA expressions were measured by quantitative RT-PCR in Bel-7404 cells treated with Huaier (15mg/ml) for 24h. Data was represented with GraphPad Prism 5.02. *p<0.05, **p<0.01 compared with control group. B. The protein expression of YAP1 in L-02, Bel-7404, Bel-7402, SMMC-7721 and HepG2 cells. C. Cell viabilities were measured by CCK-8 in Bel-7404-control and Bel-7404-YAP5SA cells with or without Huaier treatment (15mg/ml) for 24h. Data was represented with GraphPad Prism 5.02. **p<0.01 compared with control group. D. Wound healing assay was performed to test the effects of YAP5SA on migration of Bel-7404 cells with or without Huaier treatment (15mg/ml). Selecting the same point of view to take a picture at 0, 12 and 24h, and the relative scratch area was measured by ImageJ 1.74v. Data was represented with GraphPad Prism 5.02. *p<0.05 compared with control group. E. Representative IHC images of YAP1 staining from TMA analysis.