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. 2017 Nov 6;8(19):4087–4097. doi: 10.7150/jca.21018

Figure 4.

Figure 4

Huaier down-regulated YAP1 in HCC cells. A. The protein expressions of YAP/TAZ and p-YAP1 (ser127) in Bel-7404 and SMMC-7721 cells treated with Huaier at the concentrations of 0, 5, 10 and 15 mg/ml for 24h. Data was represented with GraphPad Prism 5.02. *p<0.05, **p<0.01, ***p<0.01 compared with control group. B. The ratio between p-YAP1 (ser127) and YAP. Data was represented with GraphPad Prism 5.02. *p<0.05, **p<0.01, ***p<0.01. C. Effects of CHX (100μg/ml) for 0, 1, 2, 4, 8 and 24h on YAP expression in Bel-7404 cells treated with or without Huaier (15mg/ml) for 24h. Data was represented with GraphPad Prism 5.02. D. Effects of Huaier on YAP1 in tumors as determined by immunohistochemical analysis. The representative IHC images were taken at a magnification of 20×. E. Immunefluorescence shown Huaier treatment (15mg/ml) for 24h translocated YAP1 from nucleus to cytoplasm in Bel-7404 and SMMC-7721 cells. The representative IF images were represented by LASV4.5, 100μm.