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. 2017 Nov 6;8(19):4125–4140. doi: 10.7150/jca.21802

Figure 2.

Figure 2

Figure 2

KPNB1 expression was decreased by PLK1 inhibition. (a) The expression levels of KPNB1 in H1299 cells (rpkm), which was obtained from the sequence data. (b) The relative KPNB1 levels of H1299 cells calculated with real-time PCR. RNA was extracted 48 and 72 hours after the transfection. (c) The protein expression levels of PLK1-silenced H1299 cells were detected by Western blotting. Protein was extracted 48 and 72 hours after the transfection of siRNA. (d) The correlation between PLK1 and KPNB1 expression levels of 25 lung adenocarcinoma cell lines. (e) The protein expression levels of PLK1-silenced H1299 cells were determined by Western blotting analysis. Protein was extracted 48 hours after transfection of siRNAs; each siRNA has a different sequence. (f) The protein expression levels of PLK1-silenced HeLa and A549 cells were examined by Western blotting analysis. Protein was extracted 48 hours after the transfection of siRNA. (g) The relative PLK1 levels of H1299 cells with the addition of BI2536 (6.25 or 12.5 nM) calculated with real-time PCR. RNA was extracted 48 hours after the addition of BI2536 to cells. (h) The relative KPNB1 levels of H1299 cells with the addition of BI2536 (6.25 or 12.5 nM) calculated with real-time PCR. RNA was extracted 48 hours after the addition of BI2536 to cells. (i) The protein expression levels of H1299 cells with the addition of BI2536 (10 nM) were determined by Western blotting analysis. Protein was extracted 48 hours after the addition of BI2536 to cells. Rpkm is the abbreviation for “reads per kilobase per million.” The data are given as means ± standard deviations (n = 6 replicates; *P < 0.01).