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. 2017 Nov 6;8(19):4125–4140. doi: 10.7150/jca.21802

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KPNB1 depletion induced apoptosis with the silencing of PLK1. (a) Measuring caspase-3 and -7 activities in H1299 cells 24, 48, and 72 hours after the transfection of siRNA. (b) Flow cytometry analysis of Annexin V and PI labeling of H1299 cells. The cells were harvested 48 hours after the transfection of siRNA. (c) Quantification of ATP in H1299 cells 24, 48, and 72 hours after the transfection of siRNA. (d) C1qbp expression levels of H1299 cells (rpkm). The data were acquired using a next-generation sequencer. (e) The protein expression levels of H1299 cells were detected by Western blotting analysis. The protein was extracted 48 hours after the transfection of PLK1 or control siRNA. (f) The protein expression levels of H1299 cells were determined by Western blotting analysis. The protein was extracted 48 hours after the transfection of KPNB1 or control siRNA. Rpkm is the abbreviation for “reads per kilobase per million.” Error bars represent standard deviations.

KPNB1 depletion induced apoptosis with the silencing of PLK1. (a) Measuring caspase-3 and -7 activities in H1299 cells 24, 48, and 72 hours after the transfection of siRNA. (b) Flow cytometry analysis of Annexin V and PI labeling of H1299 cells. The cells were harvested 48 hours after the transfection of siRNA. (c) Quantification of ATP in H1299 cells 24, 48, and 72 hours after the transfection of siRNA. (d) C1qbp expression levels of H1299 cells (rpkm). The data were acquired using a next-generation sequencer. (e) The protein expression levels of H1299 cells were detected by Western blotting analysis. The protein was extracted 48 hours after the transfection of PLK1 or control siRNA. (f) The protein expression levels of H1299 cells were determined by Western blotting analysis. The protein was extracted 48 hours after the transfection of KPNB1 or control siRNA. Rpkm is the abbreviation for “reads per kilobase per million.” Error bars represent standard deviations.