Figure 6.

Pseudane-VII inhibited LPS-induced MAPK phosphorylation and NF-κB translocation. RAW 264.7 cells were starved in serum-free DMEM and pre-treated with 6 μM pseudane-VII for 2 h. Cells were then stimulated with 200 ng/mL LPS for indicated times. Total cell lysates were used for MAPK detection (A), and nuclear fraction/cytosolic fractions were used to detect the NF-κB-p65 subunit (CF: Cytosol Fraction; and NF: Nucleus Fraction) (B). Equal amounts of proteins were separated by SDS-PAGE and transferred to PVDF membranes that were probed with antibodies. β-actin and histone-H3 were used as internal controls. The results are from one experiment representative of four performed that showed similar patterns.