Table 3.
Comparison of culture conditions before and after optimization.
| Compared Factors | Before Optimization * | After Optimization |
|---|---|---|
| C/N source | NB | deCSP |
| Cultivation temperature (°C) | 30 | 30 |
| C/N Concentration (%) | 0.8 | 1.6 |
| Cultivation time (day) | 4 | 4 |
| Medium/flask volume ratio | 100/250 | 130/250 |
| Seed culture (%) | 1 | 2 |
| Inhibition (IC50 μg/mL) | 81 ± 4.3 | 6.7 ± 0.31 |
| aGI productivity (U/mL) | 5000 | 12,400 |
* The optimal cultivation condition, the IC50 value and aGI productivity of FNB against S. cerevisiae α-glucosidase were obtained from a previous study [2].