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. 2017 Oct 17;2(11):1730–1736. doi: 10.1021/acssensors.7b00695

Figure 5.

Figure 5

(A) Response of 100 pM CR-LUMABS, complexed to 10 nM anticreatinine antibody, to increasing concentrations of creatinine. (B) Emission ratio of 100 pM CR-LUMABS complexed to 10 nM anticreatinine antibody followed in time. At t = 4 min, 1 mM creatinine was added. Measurements in (A) and (B) were performed in a buffer composed of 50 mM Tris, 100 mM NaCl, 10% (v/v) glycerol, and 0.05% Tween-20 at pH = 7.4. (C) Response of 100 pM CR-LUMABS, complexed to 10 nM antibody, to increasing concentrations of creatinine in 1:1 diluted blood plasma. Error bars show mean ± s.e.m. (n = 3). Solid line represents fit to eq 2.