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Epitope mapping of serum IgG against ADL. Peptide microarrays contain the ADL sequence split into 15-mer peptides with an offset of 2 amino acids. A) Epitope mapping of anti-ADL IgG from ADL-treated patients. B) Individual anti-ADL IgG epitope binding pattern of ADL-treated patients. C) IgG epitopes on ADL are located in TNF-α binding regions. For each epitope, the minimal overlapping peptide sequences are indicated. ADL interaction with TNF-α drawn with pymol (Schrödinger).
