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. 2017 Oct 17;7(19):4722–4734. doi: 10.7150/thno.21527

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Fig 1 — Staining protocol and experimental conditions. (A) Schematic of DDSI staining protocol and imaging approach. The parameters for each step of the staining protocol are listed in their respective boxes. The dual-stain soak experimental parameters that were optimized are highlighted in red. DDSI image processing was performed by subtracting the untargeted image from the targeted image and then dividing by the untargeted image as shown. (B) Table of experimental conditions tested to optimize the staining protocol.