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Temperature dependence of the excess heat capacity function of HEWL at different pH values in the range pH 2.0–6.5, where the denaturation transition is reversible. The protein concentration was ~ 1 mg/mL in all scans. The midpoint of the transition (Tm) increases with increasing pH and reaches a maximum at pH 4.5 after which a slight decrease is observed as shown in the plot of Tm as a function of pH (inset)
