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Chemical denaturation (red) and renaturation (green) curves for HEW lysozyme using GdnHCl as denaturant. For renaturation, protein was first dissolved in 20 mM bicine buffer (pH 9.0) containing 7 M GdnHCl and then diluted using buffer with varying amounts of denaturant to achieve the desired concentrations of GdnHCl. The final protein concentration was 96 μg/mL for both curves. See “Materials and Methods” for details [Color figure can be viewed at wileyonlinelibrary.com]
