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. 2017 Nov 29;18:199. doi: 10.1186/s12931-017-0677-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The immunofluorescent expression of epithelial cells (Clara cells, ciliated cells) and airway mucus. Examples of CCSP-positive Clara cells (green) and their group data are illustrated in (a) and (c) with the bar equal to 100 μm, while examples of Tubulin-positive ciliated cells (red) and their group data in (b) and (d). V:SA is the ratio of the volume to the surface area. In C, results are expressed as mean ± SEM. Numbers of mice were six in each group; * P < 0.05, HDM_Intact versus Ctrl_Intact group. And P = 0.052, HDM_PCFX versus Ctrl_PCFX group. e AB-PAS stain (bars = 200 μm). Images are representative micrographs of lung tissues obtained from each group