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. 2017 Nov 28;18:915. doi: 10.1186/s12864-017-4294-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overview of the workflow developed with the tools applied at each step (in bold). a Metagenomic reads are assembled using three assemblers: SPAdes, MetaVelvet, and IDBA. b Optimization of assembly tool using assembly statistics. c Assembled contigs from optimal assembly were binned using: MetaBat, and GroopM. d Optimal binning tool selected through bin validation. Colors (black, dark grey, and light grey) depict different microbial species, each line of a color representing the sequence belonging to a bacterial species