Table 1.
Primers used for RT-PCR and qRT-PCR analysis
| Gene | Forward primer | Reverse primer |
|---|---|---|
| GAPDH | TCCTCTGACTTCAACAGCGA | GGGTCTTACTCCTTGGAGGC |
| CCNA2 | CAGCCAGACATCACTAACAGT | CCCACAAGCTGAAGTTTTCCT |
| CCNB1 | GTTGGTGTCACTGCCATGTT | TGGCCAAAGTATGTTGCTCG |
| CCND1 | GTCTGCGAGGAACAGAAGTG | GGATGGAGTTGTCGGTGTAG |
| CCNE1 | GGAAGAGGAAGGCAAACGTG | GCAATAATCCGAGGCTTGCA |
| OCT4 | GACAGGGGGAGGGGAGGAGCTAGG | CTTCCCTCCAACCAGTTGCCCCAAAC |
| c-MYC | AGAAATGTCCTGAGCAATCACC | AAGGTTGTGAGGTTGCATTTGA |
| REX1 | ACCAGCACACTAGGCAAACC | TTCTGTTCACACAGGCTCCA |
| KLF4 | ATAGCCTAAATGATGGTGCTTGG | AACTTTGGCTTCCTTGTTTGG |
| NANOG | TGCAAGAACTCTCCAACATCCT | ATTGCTATTCTTCGGCCAGTT |
| SeV* | GGATCACTAGGTGATATCGAGC | ACCAGACAAGAGTTTAAGAGATATGTATC |
| KOS-tg* | ATGCACCGCTACGACGTGAGCGC | ACCTTGACAATCCTGATGTGG |
| Klf4-tg* | TTCCTGCATGCCAGAGGAGCCC | AATGTATCGAAGGTGCTCAA |
| cMYC-tg* | TAACTGACTAGCAGGCTTGTCG | TCC ACATACAGTCCTGGATGATGATG |
| SOX7 | TGAACGCCTTCATGGTTTG | AGCGCCTTCCACGACTTT |
| HAND1 | CCAGCTACATCGCCTACCTG | CCGGTGCGTCCTTTAATCCT |
| ACTA2 | GTGATCACCATCGGAAATGAA | TCATGATGCTGTTGTAGGTGGT |
| MYL2 | TACGTTCGGGAAATGCTGAC | TTCTCCGTGGGTGATGATG |
| NESTIN | CAGCGTTGGAACAGAGGTTGG | TGGCACAGGTGTCTCAAGGGTAG |
| BMP4 | CCTGTTGTGTGCCCACTGAAC | ATCTCAGCGGCACCCACAT |
*Used for SeV genome and transgene detection in cells reprogrammed using CytoTune 2.0 Sendai vectors (Thermo Scientific)
qRT-PCR quantitative reverse transcription PCR, RT-PCR reverse transcription PCR