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. 2017 Nov 29;15:50. doi: 10.1186/s12964-017-0205-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The interaction occurring between Sirt1 and Smad2 is domain-specific. a Schematic representation of full length GST-Smad2 and GST-Smad3 and different GST-Smad2 fragments. b A His-Pull-down assay was performed to determine interaction between Sirt1WT-6xHis and full-length GST-Smad2 or GST-Smad3, or GST-Smad2 fragments. Different constructs were detected by Western Blot. c Schematic representation of full length Sirt1WT-6xHis and different Sirt1-6xHis fragments. “CD”: catalytic domain. d His-Pull-down assay to determine interaction between Flag-Smad2 and Sirt1-6xHis and different Sirt1-6xHis fragments. Flag-Smad2 was expressed in HEK293T cells and purified with a flag peptide. Different constructs were detected by Western Blot. All the experiments of this figure were repeated at least three times. Representative results are shown