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Sirt1 accumulates on Smad regulating elements in response to TGFβ treatment in HaCaT cells. Cells were kept overnight in OPTIMEM culture medium and stimulated with TGFβ for 1.5 h. a and b. ChIP analysis of the SBR and TSS regions of the JUN gene (a) and the SBR and TSS regions of the PAI-1 (b) gene using antibodies against RNA PolII, phosphorylated-Smad2/3, Sirt1 and control IgG. Shown data are means and standard deviations of qPCRs performed in triplicate in a representative experiment
