FIGURE 1.
Blood vessel effective vascular permeability. (A) Intravital multiphoton microscopy demonstrates the presence of FITC-BSA in lymph node blood vasculature of a BALB/c mouse ~ 30 seconds after intravenous injection. (B) Image of the same location as A, ~14 minutes later shows FITC-BSA extravasation is most apparent in left side. C–D) Images at 30 s (C) and 14 min (D) after injection show extravasation of FITC-BSA in the interstitial space after VEGF-A treatment in different BALB/c mouse with extravasation most apparent in the top right corner. (E) EVP measurements performed in normal, histamine-treated and VEGF-A-treated lymph nodes, as well as subcutaneous blood vessels. Mean and SEM are reported. ** = P<.01 using Dunn’s multiple comparisons test (ANOVA)