Table 1.
20-nt spacer sgRNA
| |||
---|---|---|---|
substrate | rate constant (min−1) | S.D.a (min−1) | goodness of fit (R square) |
dsDNA + PAM | 0.104 | 0.005 | 0.9852 |
T(−1)C bulge dsDNA + PAM | 0.179 | 0.007 | 0.9904 |
T(−1)C bulge dsDNA − PAM | 0.098 | 0.009 | 0.9349 |
ssDNA | 0.65 | 0.10 | 0.8348 |
Supercoiled DNA plasmid (37 °C) | 0.093 | 0.003 | 0.9950 |
Supercoiled DNA plasmid (50 °C) | 0.26 | 0.02 | 0.9761 |
Gyrase-treated supercoiled DNA plasmid (50 °C) | 0.30 | 0.02 | 0.9649 |
BamHI-treated linearized DNA plasmid (50 °C) | 0.10 | 0.01 | 0.9484 |
24-nt spacer sgRNA
| |||
---|---|---|---|
substrate | rate constant (min−1) | S.D.a (min−1) | goodness of fit (R square) |
Supercoiled DNA plasmid (37 °C) | 0.28 | 0.01 | 0.9538 |
Supercoiled DNA plasmid (50 °C) | 1.04 | 0.03 | 0.9684 |
BamHI-treated linearized DNA plasmid (50 °C) | 0.53 | 0.05 | 0.9798 |
SD denotes standard deviation of experiments in triplicate.