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. 2017 Nov 8;11:31. doi: 10.3389/fnint.2017.00031

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Copyright © 2017 Ren, Chen, Hultborn, Guo, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double-immunostaining indicates that AADC neurons did not express ChAT or VAChT in different parts of the spinal gray matter. (A1–A3) In the DH. (B1–B3) Around the CC. (C1–C3) In the intermediate zone/ventral horn. In (C3) a ChAT-immunonegative AADC cell was located in a cluster of ChAT-immunoreactive (IR) motoneurons. (D1–D3) In the ventral horn VAChT-IR neurons did not express AADC. All images were from transverse sections at lumbar (L) segments. Arrows in different panels indicate AADC-IR cells. Cells colored red are ChAT- or VAChT-IR cells. AADC antibody was from rabbit. Scale bar in (B1), valid for (A1–B3), 50 μm; in (C1), valid for (C1–C3), and in (D1), valid for (D1–D3), 100 μm.