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. 2017 Jun 29;21(12):3453–3466. doi: 10.1111/jcmm.13255

Figure 9.

Figure 9

The mechanisms of cell death in RPE after white LED exposure. Male Wistar rats aged 7 weeks (n = 4) were exposed to white LED for 4.75 hrs and 18 hrs. NE: Non‐exposed rats. (A) At the end of the exposure period, the RPE was flat‐mounted and immunostained with anti‐RIP in green and with DAPI then analysed by confocal microscopy. The pictures were taken on the upper retina 100 μm away from the optic nerve. Scale bar represents 20 μm. (B) After LED exposure, the eyes were enucleated, the RPE dissected, extracted with M‐PER buffer and loaded on the top of a 10% SDS–PAGE, transferred onto a nitrocellulose membrane and probed for anti‐RIP. Actin was used as a charge control. (C) The histogram shows a quantification of cleaved RIP expressed as a ratio to actin. Values are significantly different *=P < 0.05.